Part:BBa_K2267029:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out. This part involves the -10 binding site, the -35 binding site, and the twenty nucleotides between that constitute the lux box. With this part, LuxR functions as a acyl-homoserine lactone-dependent repressor. LuxR resonds to the HSL produced by LuxI, N-(3-oxohexanoyl)-HSL. The Lux box is positioned such that it partially overlaps the consensus -35 and -10 hexamers of an RNA polymerase binding site.

NAME SITE HOW before after plux1 -8 "delete " C plux2 -7 " delete " G plux3 -10 substitute G A plux4 -10 substitute G T plux5 -11 substitute A T plux6 -12 substitute T C plux7 -12&-11 substitute TA CT plux8 -12&-10 substitute TAG CAA plux9 -11&-10 substitute AG TT plux10 -11&-10 substitute AG TA plux11 -12&-10 substitute TAG CAT plux12 -12&-11&-10 substitute TAG CTT plux13 -12&-11&-9 substitute TAG CTA

Applications of BBa_K2267029

Promoter activated by LuxR in concert with HSL A quorum-sensing system involving LuxR, the transcriptional activator, and an acyl-homserine lactone signal regulate the lux operon in vibrio fischeri.

User Reviews

UNIQc1bb015b64ace718-partinfo-00000000-QINU UNIQc1bb015b64ace718-partinfo-00000001-QINU