Part:BBa_K2267029:Experience
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This part involves the -10 binding site, the -35 binding site, and the twenty nucleotides between that constitute the lux box. With this part, LuxR functions as a acyl-homoserine lactone-dependent repressor. LuxR resonds to the HSL produced by LuxI, N-(3-oxohexanoyl)-HSL. The Lux box is positioned such that it partially overlaps the consensus -35 and -10 hexamers of an RNA polymerase binding site.
NAME SITE HOW before after
plux1 -8 "delete
" C
plux2 -7 "
delete " G
plux3 -10 substitute G A
plux4 -10 substitute G T
plux5 -11 substitute A T
plux6 -12 substitute T C
plux7 -12&-11 substitute TA CT
plux8 -12&-10 substitute TAG CAA
plux9 -11&-10 substitute AG TT
plux10 -11&-10 substitute AG TA
plux11 -12&-10 substitute TAG CAT
plux12 -12&-11&-10 substitute TAG CTT
plux13 -12&-11&-9 substitute TAG CTA
Applications of BBa_K2267029
Promoter activated by LuxR in concert with HSL A quorum-sensing system involving LuxR, the transcriptional activator, and an acyl-homserine lactone signal regulate the lux operon in vibrio fischeri.
User Reviews
UNIQf74943c28d5f688d-partinfo-00000000-QINU UNIQf74943c28d5f688d-partinfo-00000001-QINU